Antje Baeumner, Ph.D.   Professor and Graduate Program Director   Profile and CV	318 Riley Robb   ajb23@cornell.edu   Web site
Biosensors, Bioanalytical Systems, Microfluidics, Microbiosensors, Nanofabrication, Food & Environmental & Clinical Analysis, Food and Environmental Safety, Biotechnology, Biological Engineering, Molecular and Cellular Engineering

Research Interests

My research interests focus on the field of biotechnology, with special emphasis on analytical biotechnology, whereas my teaching will cover selected aspects of the entire field of biotechnology. In analytical biotechnology, chemical and biological techniques are combined and integrated into innovatively engineered devices for the detection of hazardous biological and chemical substances in the environment, in food, and in medical diagnostics. I am developing electrochemical and optical biosensors, e.g., for the direct extra-laboratory detection of pathogenic microorganisms, pesticides and natural toxins. Molecular biological techniques are also involved in the development of these biosensors for the detection of pathogens such as Cryptosporidium parvum. Microfabrication techniques are currently used for the preparation of signal transducers, and microfluidic devices (µTAS, micro Total Analytical Systems) will play a major role in the development of novel biosensing systems that will not only serve as easy-to-use sensing devices but will also allow their incorporation into more complex automated systems.

Professional CV

Education

• M.S., Technische Universität Braunschweig: Biotechnology, 1994
• Ph.D., Universität Stuttgart: Technical Biochemistry, 1997

Professional Experience

• Professor, Cornell University: Biological and Environmental Engineering, (11/08 - present)
• Associate Professor, Cornell University: Biological and Environmental Engineering, (11/04 - 10/08)
• Assistant Professor, Cornell University: Agricultural and Biological Engineering, (7/99 - 10/04)
• Postdoctoral Fellow, Cornell University: Food Science &Technology, (7/97 - 6/99)

Teaching Experience

• Developed Principles of Biological Engineering (BEE 260), 2004
• Developed Biosensors and Bioanalytical Techniques (BEE 459/659), 2002
• Developed Biotechnology: Principles and Applications in Engineering (BEE 458), 2001
• Organized the first practical courses for Analytical Biotechnology at the Institute for Technical Biochemistry, University of Stuttgart, 1995, 1996
• Organized an international practical course with partner groups in Stuttgart, the United Kingdom and Japan, 1995
• Supervised two masters course theses and three student research projects, University of Stuttgart, 1995-1997

International Research Activities

• Institute for Biotechnology, Cambridge University, UK, 1993
• Dept. of the Environment, Center of Research, Casacchia, ENEA, Rome, Italy, 1996
• Analytical Chemistry Labs, Department of Food Science & Technology, Cornell University, Geneva, NY, USA, 1997 -1999

Professional Societies

• American Chemical Society
• AOAC International
• Institute of Biological Engineering
• International Association of Environmental Analytical Chemistry (IAEAC)
• Society for Electroanalytical Chemistry
• Sigma Xi
• Fellow of the Gottlieb-Daimler-und-Carl-Benz-Stiftung
• Fellow of the German Academic Exchange Institution (DAAD) 

Sabbatical Leave

• University of Dortmund, Humboldt Research Fellowship, "in vivo imaging of cancer cells using nanovesicles", 2007-2008

Professional Activities

• Member of the Seneca Army Depot (NY) Restoration Advisory Board, 1998 - present
• International Advisory Committee for the Editorial Board of Analytical and Bioanalytical Chemistry (Springer), 2005-2008
• Editorial Board of Biosensors and Bioelectronics (Elsevier), 2005-2008
• Editorial Board Environmental Bioindicators (Taylor & Francis), 2005-2008
• Extended Executive Committee (EEC) for the IAEAC, 2006-2010

Technical Competence

• Biosensors
• Immunoassays
• Liposome technology
• Protein purification
• Protein immobilization
• Bacterial cell culture
• Small scale fermentation
• NASBA
• ECL
• Electrochemistry
• Screen printing for thick film technology
• Photolithography for thin film microfabrication
• Scanning electron microscopy
• UV-VIS spectroscopy
• Organic synthesis of haptens, immunogens, lipid conjugates
• Electrophoresis
• HPLC

Experienced In

• Analytical Biotechnology
• Molecular Biology
• Microbiology
• Immunochemistry
• Electrochemistry
• Microfabrication

Professional Honors

• Reinhard Böhm Award (Braunschweig University, FRG) for independent research, 1993
• Daimler-Benz Award for research at Cambridge University, UK, 1994
• 2nd Workshop on Biosensors and Biological Techniques, Lund, Sweden
• DAAD Fellowship for research at Cornell University, USA, 1997
• Cornell College of Ag. & Life Sciences Inaugural Award for Excellence in Mentoring Undergraduate Students in Independent Research, 2001
• Cornell College of Engineering Excellence in Teaching Award, 2002
• Cornell College of Engineering Excellence in Teaching Award, 2006
• Elected Vice-Co Chair for Gordon Research Conference "Bioanalytical Sensors", 2006

Publications

• Nugen, S.R., Leonard, B., Baeumner, A.J. “Application of a unique server-based oligonucleotide probe selection tool toward a novel biosensor for the detection of Streptococcus pyogenes” Biosensors and Bioelectronics (available online, 11/29/2006))
• Katie A. Edwards, Antje J. Baeumner “Synthesis of a liposome incorporated 1-carboxyalkylxanthine-phospholipid conjugate and its recognition by an RNA aptamer” Talanta, vol 71(1) pp. 365 – 372 (2007), doi:10.1016/j.talanta.2006.04.031
• Edwards, K.A. and Baeumner, A.J. “Optimization of DNA-tagged Dye- Encapsulating Liposomes for Lateral-Flow Assays Based on Sandwich-Hybridization” Analytical and Bioanalytical Chemistry vol. 386 (5), pp. 1335 – 1343 (2006) (http://dx.doi.org/10.1007/s00216-006-0705-x)
• Edwards, K.A. and Baeumner, A.J. “Optimization of DNA-tagged Liposomes for Use in Microtiter Plate Analyses” Analytical and Bioanalytical Chemistry, vol. 386 (6), pp. 1613 – 1623 (2006) (doi: 10.1007/s00216-006-0743-4)
• Li, Dapeng; Frey, Margaret W.; Baeumner, Antje J. Electrospun polylactic acid nanofiber membranes as substrates for biosensor assemblies. Journal of Membrane Science (2006), 279(1-2), 354-363.
• Vasiliy N. Goral, Natalya V. Zaytseva, Antje J. Baeumner “Electrochemical microfluidic biosensor for the detection of nucleic acid sequences” Lab-on-Chip, vol 6(3), pp. 414 - 421 (2006) DOI 10.1039/b513239h
• Katie A. Edwards and Antje J. Baeumner “A Sequential Injection Analysis System for the Sandwich-Hybridization-Based Detection of Nucleic Acids” Analytical Chemistry,vol. 78 (6), pp. 1958-1966 (2006)
• Sylvia Kwakye, Vasiliy N. Goral and Antje J. Baeumner “Electrochemical microfluidic biosensor for nucleic acid detection with integrated minipotentiostat.” Biosensors and Bioelectronics, vol. 21, pp. 2217 – 223 (2006)
• Kevin P. Nichols, Julia R. Ferullo, Antje J. Baeumner “Recirculating Microfluidic Mixer” Lab-on-Chip, vol. 6(2) pp. 242 - 246 (2006)
• Katie A. Edwards, Harriet A. Clancy and Antje J. Baeumner “Bacillus anthracis: Toxicology, Epidemiology and Current Detection Methods” Analytical and Bioanalytical Chemistry., vol. 384, pp. 73-84 (2006)
• Katie A. Edwards and Antje J. Baeumner “Analysis of Liposomes” Talanta, vol. 68 (5) pp. 1432-1441 (2006)
• Katie A. Edwards and Antje J. Baeumner “Liposomes in Analysis” Talanta, vol. 68 (5) pp. 1421-1431 (2006)
• Natalya V. Zaytseva, Richard A. Montagna, and Antje J. Baeumner “Microfluidic biosensor for the serotype-specific detection of Dengue virus” Analytical Chemistry, vol. 77, p. 7520 – 7527 (2005)
• Natalya V. Zaytseva, Vasiliy N. Goral, Richard A. Montagna, and Antje J. Baeumner “Development of a microfluidic biosensor module for pathogen detection” Lab-on-chip, vol 5 (8), pp. 805 – 811 (2005) (issue cover picture)
• Hsiao-Wei Wen, · Wlodzimierz Borejsza-Wysocki, Thomas R. DeCory, Antje J. Baeumner Richard A. Durst, “A novel extraction method for peanut allergenic proteins in chocolate and their detection by a liposome-based lateral flow assay” European Food Research and Technology, vol. 221, pp. 564 – 569 (2005)
• Chien-Sheng Chen, Antje J. Baeumner, Richard A. Durst “Protein G-liposomal nanovesicles as universal reagents for immunoassays” Talanta, vol. 67, pp. 205 – 211 (2005)
• Antje Baeumner “Bioanalytical Microsystems: Technology and Applications” invited book chapter, chapter 6, pp. 251-284 “Biosensors and Modern Biospecific Analytical Techniques”, (Ed. L. Gorton), Vol. XLIV, Comprehensive Analytical Chemistry (Ser. Ed. D. Barcelo, Elsevier, Amsterdam, 2005. doi:10.1016/S0166-526X(05)44006-4
• Antje J. Baeumner, Barbara Leonard, John McElwee, Richard A. Montagna “A Rapid Biosensor for Viable B. anthracis Spores” Analytical Bioanalytical Chemistry, vol. 380 (1), p. 15 – 23 (2004)
• Natalya Zaytseva, Richard A. Montagna, Eun Mi Lee and Antje J. Baeumner “Multi-Analyte Single-Membrane Biosensor for Serotype Specific Detection of Dengue Virus” Analytical Bioanalytical Chemistry, vol. 380 (1), p. 46 – 53 (2004)
• Antje J. Baeumner, Caroline Jones, Ching Yee Wong, and Andrew Price “A Generic Sandwich-type Biosensor with Nanomolar Detection Limits” Analytical and Bioanalytical Chemistry, vol. 378 (6), pp. 1587 – 1593 (2004)
• A.J. Baeumner, J. Pretz, S. Fang “A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits”, Analytical Chemistry, vol. 76 (4), pp. 888 – 894 (2004)
• Antje Baeumner “Bioanalytical Microsystems: Technology and Applications” invited book chapter, Editor Lo Gorton (Elsevier) accepted in Oct. 2002
• A.J. Baeumner “Biosensors for Environmental Pollutants and Food Contaminants” Analytical and Bioanalytical Chemistry, vol 377 (3), pp. 434 – 445, (2003) (invited review)
• J.-H. Min and A.J. Baeumner “Characterization and Optimization of Interdigitated Ultramicroelectrode Arrays as Electrochemical Biosensor Transducers” Electroanalysis, May 2003 (published online, Feb. 20th, 2004)
• S. Kwakye and A. Baeumner “A Microfluidic Biosensor Based on Nucleic Acid Sequence Recognition” Analytical and Bioanalytical Chemistry, vol. 376 (7), pp. 1062 – 1068 (2003)
• A.J. Baeumner and R.A. Durst “Foreword: 5th Workshop on Biosensors and Biological Techniques in Environmental Analysis” Analytica Chimica Acta, vol. 487 (1), p. 1, 2003
• H.A. Hartley and A.J. Baeumner “Biosensor for the Specific Detection of a Single Viable B. anthracis Spore” Analytical and Bioanalytical Chemistry, vol. 376 (3), pp. 319 – 327 (2003) (article published in category “Paper in Forefront”)
• S. Ahn-Yoon, R.R. DeCory, A.J. Baeumner and R.A. Durst “Ganglioside-Liposome Immunoassay for the Ultrasensitive Detection of Cholera Toxin”, Analytical Chemistry, vol. 75, pp. 2256 – 2261 (2003)
• J.-H. Min and A.J. Baeumner, “The micro-Total Analytical System for the Detection of Bacteria/Viruses” J. Industrial Engineering Chemistry, vol. 9 (1), pp. 1 – 8 (2003)
• A.J. Baeumner, R.N. Cohen, V. Miksic, J.H. Min “RNA Biosensor for the Rapid Detection of Viable Escherichia coli in Drinking Water” Biosensors & Bioelectronics, vol. 8 (4) pp. 405 – 419 (2003)
• M.D. Dhawan, F. Wise, A.J. Baeumner “Development of a Laser-Induced Cell Lysis System” Analytical and Bioanalytical Chemistry, vol. 374, pp. 421 – 426 (2002) 
• A.J. Baeumner, N. A. Schlesinger, N. S. Slutzki, J. Romano, E.M. Lee, R.A. Montagna “A biosensor for Dengue Virus Detection: Sensitive, Rapid and Serotype specific” Analytical Chemistry, vol. 74 (6), p. 1442 – 1448 (2002)
• J.-H. Min and A.J. Baeumner “Highly Sensitive and Specific Detection of Viable Escherichia coli in Drinking water” Analytical Biochemistry, vol. 303, p. 186 – 193 (2002)
• M.B. Esch, A.J. Baeumner, R.A. Durst “Detection of Cryptosporidium parvum Using Oligonucleotide-Tagged Liposomes in a Competitive Assay Format “Analytical Chemistry vol. 73 (13), pp. 3162-3167 (2001)
• A.J. Baeumner, M.C. Humiston, R.A. Montagna, R.A. Durst “Detection of Viable Oocysts of Cryptosporidium parvum Following Nucleic Acid Sequence-Based Amplification” Analytical Chemistry vol. 73 (6), pp. 1176-1180 (2001)
• L. Mosiello, L. Segre, S. Chiavarini, C. Cremisini, M. Spano, A.J. Bäumner, T. Kimmel, R.D. Schmid “Dipstick Immunoassay Format for Atrazine and Terbutylazine Analysis in Water Samples.” J. Agricultural and Food Chemistry vol. 46 (9), 3847 - 3851, 1998
• A.J. Bäumner and R.D. Schmid, “Development of a New Immunosensor for Pesticide Detection: a Disposable System with Liposome-Enhancement and Amperometric Detection” Biosensors & Bioelectronics vol. 13 (5), 519 - 529 (1998)
• R.A. Durst, A.J. Bäumner, R.W. Murray, R.P. Buck, C.P. Andrieux, “Chemically Modified Electrodes: Recommended Terminology and Definitions” Pure & Applied Chemistry vol. 69 (6), pp. 1317 - 1323 (1997)
• A.J. Bäumner, T. Kummer, R.D. Schmid, “Liposome-Based Immunosensors: 1. Influence of Hapten Spacer Length on Liposome Binding Efficiency.” Analytical Letters vol 29 (15), pp. 2601-2613 (1996)

Non-peer Reviewed

• A.J. Baeumner and R.A. Durst “Liposome-amplified bioanalytical techniques” LabPlus International, Oct. 2004, p. 21 – 25 (2004)
• A.J. Baeumner “Nanosensors Identify Pathogens in Food” Food Technology (Feature Article) Vol. 58 (8), p. 51 – 55 (2004)
• J. H. Cunningham and A.J. Baeumner “Biosensors for the detection of pathogenic organisms” LabPlus International, Nov. 2001, Vol. 15(5), 2001

Patents Issued

1. Title: Liposome-Enhanced Test Device and Method Inventorship: Richard A. Durst, Richard A. Montagna, Antje J. Baeumner, Sui T. Siebert, Geoffrey Rule US patent # 6,358,752 CRF Ref#: D-2323B Country: USA Date Filed: May 20, 1999, Date Issued: March 19, 2002
2. Title: Filtration-Detection Device and Method of Use Inventorship: Antje J. Baeumner US patent #: 6,576,460 CRF Ref #: D-2560A Country: USA Date Filed: October 27, 2000, allowed, Sept. 2002, Date Issued: June 6th, 2003
3. Title Development of a laser-induced cell lysis system Inventorship A. J. Baeumner, M. D. Dhawan US patent #: 6,815,209 CRF Ref. # D-2970 Country USA Date Filed Nov. 16th, 2002 Date issued Nov. 9th, 2004

Patents Filed

1. Title Universal Biosensor Inventorship Antje J. Baeumner Application # 10/449,369 CRF Ref. # D-3077 Country worldwide Date Filed May 30th, 2003
2. Title ELECTROSPUN NANOFIBER-BASED BIOSENSOR ASSEMBLIES Inventorship Margarete Frey, Antje J. Baeumner,Dapeng Li, Application # CRF Ref. # D-3690 Country Date Filed May 23rd, 2005
3. Title Recirculating Microfluidic Mixer Inventorship Antje J. Baeumner, Kevin P. Nichols Application # 60/689,720 CRF Ref. # D-3607-01 Country Date Filed June 10th, 2005